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KMID : 1035520140020010022
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Brain Tumor Research and Treatment : BTRT
2014 Volume.2 No. 1 p.22 ~ p.28
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Proteomic Analysis between U87MG and U343MG-A Cell Lines: Searching for Candidate Proteins for Glioma Invasion
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Pei Jian
Moon Kyung-Sub
Pan SangO
Lee Kyung-Hwa
Ryu Hyang-Hwa
Jung Tae-Young
Kim In-Young
Jang Woo-Yeol
Jung Chae-Hun
Jung Shin
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Abstract
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Background: To investigate the molecular basis for invasion of malignant gliomas, proteomic analysis approach was carried out using two human glioma cell lines, U87MG and U343MG-A that demonstrate different motility and invasiveness in in vitro experiments.
Methods: High-resolution two-dimensional gel electrophoresis and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry analysis were performed.
Results: Nine distinct protein spots that were recognized with significant alteration between the two cell lines. Five of these protein spots were up-regulated in U87MG and four were up-regulated in U343MG-A.
Conclusion: Among these proteins, cathepsin D was shown to be one of the important proteins which are related with glioma invasion. However, further studies are necessary to reveal the exact role and mechanism of cathepsin D in glioma invasion.
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KEYWORD
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Cathepsin D, Gliomas, Invasion, Motility, Protein, Proteomics
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